Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Rapid Detection of Isolates Belonging to the Epidemic Clones Achromobacter xylosoxidans ST137 and Achromobacter ruhlandii DES from Cystic Fibrosis Patients

• Published in: Journal of clinical microbiology Vol. 59; no. 10; p. e0094621
• Main Authors: Garrigos, Thomas, Dollat, Manon, Magallon, Arnaud, Chapuis, Angélique, Varin, Véronique, Bador, Julien, Makki, Nadia, Cremet, Lise, Persyn, Elise, Cardot-Martin, Emilie, Echahidi, Fedoua, Peeters, Charlotte, Pierard, Denis, Vandamme, Peter, Verroken, Alexia, Neuwirth, Catherine, Amoureux, Lucie
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 20-09-2021
• Subjects: Achromobacter; Achromobacter denitrificans - genetics; Bacteriology; Clone Cells; Cystic Fibrosis - complications; Cystic Fibrosis - epidemiology; Epidemics; Humans; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Danish epidemic strain; epidemic clones; MALDI-TOF MS; Achromobacter ruhlandii; cystic fibrosis; typing; Achromobacter xylosoxidans; ST137
• ISSN: 0095-1137; 1098-660X
• DOI: 10.1128/JCM.00946-21

spp. are increasingly reported among cystic fibrosis patients. Genotyping requires time-consuming methods such as multilocus sequence typing or pulsed-field gel electrophoresis. Therefore, data on the prevalence of multiresistant epidemic clones, especially A. xylosoxidans ST137 (AxST137) and the Danish epidemic strain
(DES), are lacking. We recently developed and published a database for
species identification by matrix-assisted laser desorption-ionization-time of flight mass spectrometry (MALDI-TOF MS; Bruker Daltonics). The aim of this study was to evaluate the ability of the MALDI-TOF MS to distinguish these multiresistant epidemic clones within
species. All the spectra of A. xylosoxidans (
 = 1,571) and
(
 = 174) used to build the local database were analyzed by ClinProTools, MALDI Biotyper PCA, MALDI Biotyper dendrogram, and flexAnalysis software for biomarker peak detection. Two hundred two isolates (including 48 isolates of AxST137 and 7 of DES) were tested. Specific biomarker peaks were identified: absent peak at
6,651 for AxST137 isolates and present peak at
9,438 for DES isolates. All tested isolates were well typed by our local database and clustered within distinct groups (ST137 or non-ST137 and DES or non-DES) no matter the MALDI-TOF software or only by simple visual inspection of the spectra by any user. The use of MALDI-TOF MS allowed us to identify isolates of A. xylosoxidans belonging to the AxST137 clone that spread in France and Belgium (the Belgian epidemic clone) and of
belonging to the DES clone. This tool will help the implementation of segregation measures to avoid interpatient transmission of these resistant clones.