A Semisynthetic Fluorescent Sensor Protein for Glutamate

We report the semisynthesis of a fluorescent glutamate sensor protein on cell surfaces. Sensor excitation at 547 nm yields a glutamate-dependent emission spectrum between 550 and 700 nm that can be exploited for ratiometric sensing. On cells, the sensor displays a ratiometric change of 1.56. The hig...

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Bibliographic Details
Published in:	Journal of the American Chemical Society Vol. 134; no. 18; pp. 7676 - 7678
Main Authors:	Brun, Matthias A, Tan, Kui-Thong, Griss, Rudolf, Kielkowska, Anna, Reymond, Luc, Johnsson, Kai
Format:	Journal Article
Language:	English
Published:	United States American Chemical Society 09-05-2012
Subjects:	Biosensing Techniques - methods Cell Membrane - chemistry Cell Membrane - metabolism Fluorescent Dyes - chemistry Gene Expression Glutamic Acid - analysis Glutamic Acid - metabolism HEK293 Cells Humans Protein Structure, Tertiary Receptors, Glutamate - chemistry Receptors, Glutamate - genetics Receptors, Glutamate - metabolism Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Sensitivity and Specificity
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Summary:	We report the semisynthesis of a fluorescent glutamate sensor protein on cell surfaces. Sensor excitation at 547 nm yields a glutamate-dependent emission spectrum between 550 and 700 nm that can be exploited for ratiometric sensing. On cells, the sensor displays a ratiometric change of 1.56. The high sensitivity toward glutamate concentration changes of the sensor and its exclusive extracellular localization make it an attractive tool for glutamate sensing in neurobiology.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0002-7863 1520-5126
DOI:	10.1021/ja3002277