Changes Induced by Fe Deficiency and Fe Resupply in the Root Protein Profile of a Peach-Almond Hybrid Rootstock

• Published in: Journal of proteome research Vol. 12; no. 3; pp. 1162 - 1172
• Main Authors: Rodríguez-Celma, Jorge, Lattanzio, Giuseppe, Jiménez, Sergio, Briat, Jean-Francois, Abadía, Javier, Abadía, Anunciación, Gogorcena, Yolanda, López-Millán, Ana-Flor
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 01-03-2013
• Subjects: Chromatography, Liquid; Electrophoresis, Gel, Two-Dimensional; Iron; Iron - deficiency; Iron - metabolism; Life Sciences; Plant Proteins; Plant Proteins - metabolism; Plant Roots; Plant Roots - metabolism; Proteomics; Prunus; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry; Vegetal Biology; Prunus; iron; metabolism; root; two-dimensional gel electrophoresis
• ISSN: 1535-3893; 1535-3907
• DOI: 10.1021/pr300763c

The changes in the root extract protein profile of the Prunus hybrid GF 677 rootstock (P. dulcis × P. persica) grown in hydroponics as affected by Fe deficiency and short-term (24 h) Fe resupply have been studied by 2-dimensional gel electrophoresis-based techniques. A total of 335 spots were consistently found in the gels. Iron deficiency caused above 2-fold increases or >50% decreases in the relative abundance in 10 and 6 spots, respectively, whereas one spot was only detected in Fe-deficient plants. Iron resupply to Fe-deficient plants caused increases and decreases in relative abundance in 15 and 16 spots, respectively, and one more spot was only detected in Fe-resupplied Fe-deficient plants. Ninety-five percent of the proteins changing in relative abundance were identified using nanoliquid chromatography-tandem mass spectrometry. Defense responses against oxidative and general stress accounted for 50% of the changes in Fe-deficient roots. Also, a slight induction of the glycolysis-fermentation pathways was observed in GF 677 roots with Fe deficiency. The root protein profile of 24 h Fe-resupplied plants was similar to that of Fe-deficient plants, indicating that the deactivation of Fe-deficiency metabolic responses is slow. Taken together, our results suggest that the high tolerance of GF 677 rootstock to Fe deficiency may be related to its ability to elicit a sound defense response against both general and oxidative stress.