On-Chip Micropatterning of Plastic (Cylic Olefin Copolymer, COC) Microfluidic Channels for the Fabrication of Biomolecule Microarrays Using Photografting Methods

• Published in: Langmuir Vol. 23; no. 3; pp. 1577 - 1583
• Main Authors: Pu, Qiaosheng, Oyesanya, Olufemi, Thompson, Bowlin, Liu, Shantang, Alvarez, Julio C
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 30-01-2007
• Subjects: Biopolymers - chemistry; Chemistry; Electrochemistry; Equipment Design; Exact sciences and technology; General and physical chemistry; Microfluidics - instrumentation; Microscopy, Atomic Force; Plastics; Spectrum Analysis; Surface Properties; Ultraviolet Rays; Atomic force microscopy; Modification; Surface reaction; Transparency; Film; Electrokinetics; Thin film; Characterization; Copolymer; Monomer; Functional group; Thermoplastics; Protein; Thickness; Infrared spectrometry; Chemistry; Density functional; Olefin; Chemical reaction; DNA; Electrochemistry; Micrometer; Ethylenic compound; Fluorescence microscopy; Reflectance
• ISSN: 0743-7463; 1520-5827
• DOI: 10.1021/la062662t

This paper reports on the surface modification of plastic microfluidic channels to prepare different biomolecule micropatterns using ultraviolet (UV) photografting methods. The linkage chemistry is based upon UV photopolymerization of acryl monomers to generate thin films (0.01−6 μm) chemically linked to the organic backbone of the plastic surface. The commodity thermoplastic, cyclic olefin copolymer (COC) was selected to build microfluidic chips because of its significant UV transparency and easiness for microfabrication by molding techniques. Once the polyacrylic films were grafted on the COC surface using photomasks, micropatterns of proteins, DNA, and biotinlated conjugates were readily obtained by surface chemical reactions in one or two subsequent steps. The thickness of the photografted films can be tuned from several nanometers up to several micrometers, depending on the reaction conditions. The micropatterned films can be prepared inside the microfluidic channel (on-chip) or on open COC surfaces (off-chip) with densities of functional groups about 10-7 mol/cm2. Characterization of these films was performed by attenuated-total-reflectance IR spectroscopy, fluorescence microscopy, profilometry, atomic force microscopy, and electrokinetic methods.