Comparison of Supercritical Carbon Dioxide and Soxhlet Extraction of Lipids from a Brown Seaweed, Sargassum hemiphyllum (Turn.) C. Ag.

• Published in: Journal of agricultural and food chemistry Vol. 46; no. 10; pp. 4228 - 4232
• Main Authors: Cheung, Peter C. K, Leung, Arnold Y. H, Ang, Put O
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01-10-1998
• Subjects: ACIDE GRAS; ACIDE GRAS POLYINSATURE; ACIDOS GRASOS; ACIDOS GRASOS POLIINSATURADOS; ANALYTICAL METHODS; Biological and medical sciences; CHEMICAL COMPOSITION; Chemical constitution; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; EXTRACCION; EXTRACTION; FATTY ACIDS; Food industries; Fundamental and applied biological sciences. Psychology; LIPIDE; LIPIDOS; LIPIDS; OMEGA-3-FATTY ACIDS; PHAEOPHYCEAE; Plant physiology and development; POLYUNSATURATED FATTY ACIDS; Research and development. New food products, dietetic foods and beverages; SUPERCRITICAL CARBON DIOXIDE EXTRACTION; TECHNIQUE ANALYTIQUE; TECNICAS ANALITICAS; Soxhlet extraction; Chemical analysis; Algae; Marine plant; Carbon dioxide; Polyunsaturated fatty acid; Lipids; n-3 fatty acid; Optimization; Heterokontophyta; Chemical composition; Supercritical fluid extraction; Comparative study; Phaeophyceae; Thallophyta
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf980346h

The fatty acid profiles of lipids extracted Sargassum hemiphyllum by supercritical carbon dioxide (SC-CO2) were compared with those obtained by Soxhlet solvent extraction using chloroform/methanol. For the pressure range (24.1−37.9 MPa) and temperature interval (40−50 °C), 37.9 MPa/40 °C and 37.9 MPa/50 °C gave the highest lipid yield, which was comparable to that of the Soxhlet method, and the highest concentrations of total and individual ω-3 fatty acids, which were significantly higher (p < 0.05) than the solvent extraction. However, SC-CO2 extraction at higher temperature but low-pressure levels (24.1 MPa/50 °C) gave significantly lower (p < 0.05) lipid yield and fatty acid concentration than that of lower temperature but of the same pressure (24.1 MPa/40 °C). Concentrations of docosapentaenoic acid and docosahexaenoic acid were significantly higher (p < 0.05) at 37.9 MPa/40 °C than at 37.9 MPa/50 °C. Concentration of the total polyunsaturated fatty acids increased significantly (p < 0.05) and concentration of total saturated fatty acids decreased significantly (p < 0.05) with increasing pressure and solvent density, as indicated by the saturated/unsaturated fatty acid ratios. Keywords: Seaweed; supercritical fluid carbon dioxide; omega-3 fatty acids; lipids