Protein-Functionalized Hairy Diamond Nanoparticles

• Published in: Langmuir Vol. 25; no. 17; pp. 9633 - 9638
• Main Authors: Dahoumane, Si Amar, Nguyen, Minh Ngoc, Thorel, Alain, Boudou, Jean-Paul, Chehimi, Mohamed M, Mangeney, Claire
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01-09-2009
• Subjects: Animals; Cattle; Chemistry; Colloidal state and disperse state; Diamond - chemistry; Diazonium Compounds - chemistry; Electron Probe Microanalysis - methods; Engineering Sciences; Exact sciences and technology; Fluorescence; General and physical chemistry; Hydrolysis; Materials; Methacrylates - chemistry; Microscopy, Electron, Transmission - methods; Nanoparticles - chemistry; Nanotechnology - methods; Nitrogen - chemistry; Physical and chemical studies. Granulometry. Electrokinetic phenomena; Polymers - chemistry; Proteins - chemistry; Salts - chemistry; Serum Albumin - chemistry; Surface physical chemistry; Nanoparticle; Diamond; Protein
• ISSN: 0743-7463; 1520-5827
• DOI: 10.1021/la9009509

Diazonium salt chemistry and atom transfer radical polymerization (ATRP) were combined in view of preparing new bioactive hairy diamond nanoparticles containing, or potentially containing, nitrogen-vacancy (NV) fluorescent centers (fluorescent nanodiamonds, or fNDs). fNDs were modified by ATRP initiators using the electroless reduction of the diazonium salt BF4 −,+N2−C6H4−CH(CH3)−Br. The strongly bound aryl groups −C6H4−CH(CH3)−Br efficiently initiated the ATRP of tert-butyl methacrylate (tBMA) at the surface of the nanodiamonds, which resulted in obtaining ND-PtBMA hybrids. The grafted chain thickness, estimated from X-ray photoelectron spectroscopy (XPS), was found to increase linearly with respect to time before reaching a plateau value of ca. 2 nm. These nanoobjects were further hydrolyzed into ND-PMAA (where PMAA is the poly(methacrylic acid) graft) and further decorated by bovine serum albumin through the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) coupling procedure.