Interactions of Oxytetracycline with a Smectite Clay: A Spectroscopic Study with Molecular Simulations

• Published in: Environmental science & technology Vol. 44; no. 20; pp. 7839 - 7845
• Main Authors: Aristilde, Ludmilla, Marichal, Claire, Miéhé-Brendlé, Jocelyne, Lanson, Bruno, Charlet, Laurent
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 15-10-2010
• Subjects: Aluminum Silicates; Antibiotics; Applied sciences; Clay; Diffraction; Earth Sciences; Environmental Processes; Environmental Sciences; Exact sciences and technology; Geochemistry; Global Changes; Magnetic Resonance Spectroscopy; Minerals; Models, Molecular; Molecular chemistry; NMR; Nuclear magnetic resonance; Oxytetracycline - chemistry; Pollution; Sciences of the Universe; Silicates; Simulation; Spectrophotometry, Infrared; Spectrum analysis; X-Ray Diffraction; Drug; Complexation; Monte Carlo method; Montmorillonite; Pollutant behavior; Tetracycline derivatives; Mobility; NMR spectrometry; X ray diffraction; Modeling; Infrared spectrometry; Antibiotic; Pollution; Adsorption; Smectite; Micropollutant; Ion exchange; Organic compounds
• ISSN: 0013-936X; 1520-5851
• DOI: 10.1021/es102136y

Binding of antibiotics to clay minerals can decrease both their physical and biological availability in soils. To elucidate the binding mechanisms of tetracycline antibiotics on smectite clays as a function of pH, we probed the interactions of oxytetracycline (OTC) with Na-montmorillonite (MONT) using X-ray diffraction (XRD), infrared (IR), and solid-state nuclear magnetic resonance (NMR) spectroscopies, and Monte Carlo molecular simulations. The XRD patterns demonstrate the presence of OTC in the MONT interlayer space at acidic pH whereas complexation of OTC by external basal and edge sites seems to prevail at pH 8. At both pH, the 1H−13C NMR profile indicates restricted mobility of the adsorbed OTC species; and, −CH3 deformation and CN stretching IR vibration bands confirm a binding mechanism involving the protonated dimethylamino group of OTC. Changes in the 23Na NMR environments are consistent with cation-exchange and cation complexation reactions at the different sites of adsorption. Molecular simulations indicate that MONT interlayer spacing and structural charge localization dictate favorable binding conformations of the intercalated OTC, facilitating multiple interactions in agreement with the spectroscopic data. Our results present complementary insights into the mechanisms of adsorption of TETs on smectites important for their retention in natural and engineered soil environments.