Isolation of a Novel Endophytic Bacillus Strain Capable of Transforming Pentachlorophenol and Structure Determination of Pentachlorophenol Phosphate Using Single-Crystal X‑ray Diffraction

• Published in: Journal of agricultural and food chemistry Vol. 70; no. 3; pp. 770 - 776
• Main Authors: Ito, Koji, Kataoka, Ryota, Katayama, Shunki, Kiyota, Hiromasa, Mahmood, Ahmad, Kikuchi, Takashi, Sato, Takashi, Sakakibara, Futa, Takagi, Kazuhiro
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 26-01-2022
• Subjects: Agricultural and Environmental Chemistry; Bacillus; Biodegradation, Environmental; Pentachlorophenol; Phosphates; Soil Pollutants; X-Ray Diffraction; metabolic pathway; SCX; bioremediation; PCP phosphate; endophytic bacteria
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/acs.jafc.1c05987

A novel approach for the remediation of upland soils contaminated with pentachlorophenol (C6HCl5O; PCP) (1), a fungicide, wood perservative, and herbicide, through the exploitation of plant-endophytic bacteria may overcome the existing issues in bioaugmentaion and phytoremidiation. In this study, we isolated the endophytic Bacillus sp. strain PCP15 and determined its metabolite of PCP (1). This strain degraded 8.03 μmol L–1 PCP (1) within 24 h and generated the novel metabolite PCP phosphate (3). The PCP15 strain showed nearly complete growth inhibition of 20 μmol L−1 PCP (1). In contrast, PCP15 showed resistance to PCP phosphate (3), indicating that the phosphorylation of PCP, which has never previously been reported in organisms, contributed to the detoxification of PCP (1) in bacterial cells. Our results show the potential for practical application of this strain in hybrid remediation of PCP (1)-contaminated soils and reveal a novel PCP (1) detoxification mechanism in organisms.