Inhibition of the Proliferation and Invasion of C6 Glioma Cells by Tricin via the Upregulation of Focal-Adhesion-Kinase-Targeting MicroRNA‑7

• Published in: Journal of agricultural and food chemistry Vol. 66; no. 26; pp. 6708 - 6716
• Main Authors: Chung, Dai-Jung, Wang, Chau-Jong, Yeh, Chia-Wei, Tseng, Tsui-Hwa
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 05-07-2018
• Subjects: microRNA-7; focal-adhesion kinase; glioma; tricin; anticancer
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/acs.jafc.8b00604

Tricin, a natural flavonoid present in large amounts in rice bran, was investigated for the mechanisms by which it exhibited antiproliferation and anti-invasion in C6 glioma cells. The results indicated that treatment with 5, 10, 25, and 50 μM tricin for 48 h significantly (p < 0.05) inhibited cell numbers and colony numbers with values of 134.3 ± 5.5, 114.6 ± 2.5, 106.3 ± 3.2, and 57.3 ± 10.2, respectively. Tricin also inhibited C6-cell motility, migration, and invasion. Tricin changed the cytoskeletal organization, reduced matrix-metalloproteinase (MMP) expression, and upregulated E-cadherin. Tricin decreased FAK protein levels and suppressed focal-adhesion-kinase (FAK)-downstream-signal activation. Most importantly, tricin dose-dependently upregulated microRNA-7 (miR-7). Transfection with an miR-7 inhibitor suppressed miR-7 expression, increased FAK expression, and promoted the proliferation and invasion in C6 cells. The data support a novel anticancer mechanism of tricin that involves upregulation of FAK-targeting miR-7 in C6 glioma cells.