Backbone Cyclised Peptides from Plants Show Molluscicidal Activity against the Rice Pest Pomacea canaliculata (Golden Apple Snail)

• Published in: Journal of agricultural and food chemistry Vol. 56; no. 13; pp. 5237 - 5241
• Main Authors: Plan, Manuel Rey R, Saska, Ivana, Cagauan, Arsenia G, Craik, David J
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 09-07-2008
• Subjects: Acetaldehyde - analogs & derivatives; Acetaldehyde - chemical synthesis; Acetaldehyde - toxicity; Amino Acid Sequence; Animals; backbone cyclized peptides; Biological and medical sciences; Biological control; Cereal and baking product industries; Cichlids; Control; Crop and Animal Protection Chemistry; Cyclotides; Cyclotides - chemistry; Cyclotides - toxicity; Food industries; Fundamental and applied biological sciences. Psychology; golden apple snail (Pomacea canaliculata); Magnoliopsida - chemistry; Magnoliopsida - metabolism; metaldehyde; Molecular Sequence Data; mollusc control; Molluscacides - chemistry; Molluscacides - toxicity; molluscicidal properties; natural toxicants; Nile tilapia (Oreochromis niloticus); nontarget organisms; Oldenlandia affinis; Oryza - parasitology; peptides; Pesticides - chemistry; Pesticides - toxicity; phytochemicals; Phytopathology. Animal pests. Plant and forest protection; Plant Diseases - parasitology; plant extracts; Plant Extracts - chemistry; Plant Extracts - toxicity; plant pests; Pomacea canaliculata; Protozoa. Invertebrates; rice; rotenone; Rubiaceae; Sequence Alignment; Snails - drug effects; toxicity; Viola odorata; Violaceae; South East Asia; Viola odorata; golden apple snail (Pomacea canaliculata); Rubiaceae; rotenone; Cyclotides; Violaceae; Nile tilapia (Oreochromis niloticus); metaldehyde; Oldenlandia affinis; Insecticide; Peptides; Viola odorata; Gastropoda; golden apple snail (Pomacea canaliculata); Pest; Dicotyledones; Pisces; Angiospermae; Rice; Fruit; Pesticides; Tilapia; Snail; Vertebrata; Oreochromis niloticus; Apple; Cereal; Spermatophyta; Invertebrata; Mollusca
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf800302f

Golden apple snails (Pomacea canaliculata) are serious pests of rice in South East Asia. Cyclotides are backbone cyclized peptides produced by plants from Rubiaceae and Violaceae. In this study, we investigated the molluscicidal activity of cyclotides against golden apple snails. Crude cyclotide extracts from both Oldenlandia affinis and Viola odorata plants showed molluscicidal activity comparable to the synthetic molluscicide metaldehyde. Individual cyclotides from each extract demonstrated a range of molluscicidal activities. The cyclotides cycloviolacin O1, kalata B1, and kalata B2 were more toxic to golden apple snails than metaldehyde, while kalata B7 and kalata B8 did not cause significant mortality. The toxicity of the cyclotide kalata B2 on a nontarget species, the Nile tilapia (Oreochromis niloticus), was three times lower than the common piscicide rotenone. Our findings suggest that the existing diversity of cyclotides in plants could be used to develop natural molluscicides.