Contact Killing of Cu-Bearing Stainless Steel Based on Charge Transfer Caused by the Microdomain Potential Difference

• Published in: ACS applied materials & interfaces Vol. 12; no. 1; pp. 361 - 372
• Main Authors: Zhang, Xinrui, Yang, Chunguang, Yang, Ke
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 08-01-2020
• Subjects: surface potential; Cu(I); Cu(II); Cu-bearing stainless steel; contact killing; charge transfer
• ISSN: 1944-8244; 1944-8252
• DOI: 10.1021/acsami.9b19596

The addition of copper makes the Cu-bearing stainless steel (SS) possess excellent antibacterial properties. However, the antibacterial mechanism of the Cu-bearing SS is still not accurately understood and recognized. On the one hand, the concentration of released antibacterial Cu ions from its surface is insufficient to generate such an effect. On the other hand, due to the limited Cu content, the area of copper toxicity that can be contacted with bacteria is also much less than that of pure Cu. Therefore, the purpose of this study was to explore the way of bacterial inactivation caused by Cu-bearing SS from the view of the charge transfer. The results showed that the continuous and effective contact between bacteria and Cu-bearing SS is the key to induce the bacteria-killing effect so that the cathode electrons generated by the potential difference of the material microdomain can cause the proton depletion in the bacterial cells, thereby disturbing the respiratory chain and energy generation of the bacterial cells. The proton depletion reaction also catalyzed the conversion of Cu­(II) into Cu­(I). Cu­(I) not only destroys the iron–sulfur protein but also undergoes the redox reaction with Cu­(II) to produce reactive oxygen species, causing oxidative damage to cells, eventually accelerating the bacterial death.