Automated rate nephelometric determination of apolipoproteins AI and B in human serum by consecutive addition of antibodies

• Published in: Analytical chemistry (Washington) Vol. 64; no. 15; pp. 1698 - 1701
• Main Author: Fotiou, Fotis K
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01-08-1992
• Subjects: Analytical, structural and metabolic biochemistry; Antibodies - analysis; Apolipoprotein A-I - analysis; Apolipoprotein A-I - immunology; Apolipoproteins B - blood; Apolipoproteins B - immunology; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Humans; Immunity (Disease); Immunoassay; Indicators and Reagents; Lipoproteins, myelin; Medical research; Proteins; Apolipoprotein B; Human; Nephelometry; Analytical method; Serum; Quantitative analysis; Immunological method
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/ac00039a013

A precise, rapid, automated, rate nephelometric immunoassay for apolipoprotein AI (APA) and apolipoprotein B (APB) is described. Both analytes are determined by a "one-pot" procedure which uses consecutive addition of the corresponding antibodies. Poly(oxyethylene) type nonionic surfactants are used to selectively inhibit the APB reaction after the maximum reaction rate has been reached and to enhance the APA immunoreactivity. The assay range for APA is 0.3-3.8 g/L and for APB 0.2-3.0 g/L. The new assay was compared to commercial rate nephelometric methodology. Both methods were shown to be mutually unbiased for APA and APB determinations in human serum.