Supramolecular Self-Assembly of Glutamine Synthetase: Mutagenesis of a Novel Intermolecular Metal Binding Site Required for Dodecamer Stacking

• Published in: Biochemistry (Easton) Vol. 33; no. 50; pp. 14957 - 14964
• Main Authors: Dabrowski, Michael J, Yanchunas, Joseph, Villafranca, Beth Cader, Dietze, Eric C, Schurke, Peter, Atkins, William M
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 01-12-1994
• Subjects: Binding Sites; Cobalt - metabolism; Cobalt - pharmacology; Computer Simulation; Copper - metabolism; Escherichia coli; Escherichia coli - enzymology; Glutamate-Ammonia Ligase - chemistry; Glutamate-Ammonia Ligase - genetics; Macromolecular Substances; Magnesium - metabolism; Manganese - metabolism; Metals - metabolism; Metals - pharmacology; Microscopy, Electron; Models, Molecular; Molecular Structure; Mutagenesis, Site-Directed; Protein Structure, Secondary; Structure-Activity Relationship; Zinc - metabolism
• ISSN: 0006-2960; 1520-4995
• DOI: 10.1021/bi00254a002

Dodecameric glutamine synthetase (GS) from Escherichia coli assembles into highly ordered supramolecular protein tubes in the presence of several divalent metal ions. The molecular mechanism for this metal-induced self-assembly of the E. coli GS has been studied by molecular modeling and site-directed mutagenesis. The X-ray crystal structure of the nearly identical Salmonella typhimurium GS has been used to construct a model of the "stacked" complex between two dodecamers. A complementary fit, based on steric constraints, reveals a possible interaction between the N-terminal helices from adjacent dodecamers. The amino acid side chains of His and Met residues within the helices from each of the subunits of one face of a dodecamer lie within approximately 3.5 A of the analogous side chains in the subunits from the adjacent dodecamer in the stacked complex. His-4, Met-8, and His-12 from adjacent helices provide potential ligands for a binuclear metal binding site. Replacement of each of these surface residues with aliphatic amino acids has negligible effects on the enzymatic activity, the regulation of activity via adenylylation, and gross dodecameric structure. However, the rate and extent of metal ion-mediated self-assembly of GS tubules are reduced to < 2% of the wild-type protein in the single mutants H4A, H12L, and H12D. The M8L mutant demonstrates a 3-fold decrease in the bimolecular rate constant for stacking, but electron microscopy indicates that this mutant does form stacked tubes. The cysteine-containing mutants H4C, M8C, and H12C were also constructed.