Differential Response of Endothelial and Endothelial Colony Forming Cells on Electrospun Scaffolds with Distinct Microfiber Diameters

• Published in: Biomacromolecules Vol. 15; no. 3; pp. 821 - 829
• Main Authors: Fioretta, Emanuela S, Simonet, Marc, Smits, Anthal I. P. M, Baaijens, Frank P. T, Bouten, Carlijn V. C
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 10-03-2014
• Subjects: Applied sciences; Biological and medical sciences; Cell Adhesion; Collagen; Endothelial Cells - chemistry; Exact sciences and technology; Fibers and threads; Forms of application and semi-finished materials; Human Umbilical Vein Endothelial Cells - chemistry; Humans; Medical sciences; Polyesters - chemistry; Polymer industry, paints, wood; Polymers - chemistry; Stem Cells - chemistry; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology of polymers; Technology. Biomaterials. Equipments; Tissue Scaffolds - chemistry; Endothelial cell; Phenotype; Spatial distribution; Tissue engineering; Synthetic fiber; Biomaterial; Experimental study; Gene expression; Lactone polymer; Polycaprolactone; Framework
• ISSN: 1525-7797; 1526-4602
• DOI: 10.1021/bm4016418

Electrospun scaffolds for in situ tissue engineering can be prepared with different fiber diameters to influence cell recruitment, adhesion, and differentiation. For cardiovascular applications, we investigated the impact of different fiber diameters (2, 5, 8, and 11 μm) in electrospun poly­(ε-caprolactone) scaffolds on endothelial colony forming cells (ECFCs) in comparison to mature endothelial cells (HUVECs). In 2D cultures and on 2 μm fiber scaffolds, ECFC morphology and phenotype resemble those of HUVECs. When cultured on scaffolds with 5–11 μm fibers, a different behavior was detected. HUVECs developed a cytoskeleton organized circumferentially around the fibers, with collagen alignment in the same direction. ECFCs, instead, aligned the cytoskeleton along the scaffold fiber axis and deposited a homogeneous layer of collagen over the fibers; moreover, a subpopulation of ECFCs gained the αSMA marker. These results showed that ECFCs do not behave like mature endothelial cells in a 3D fibrous environment.