Selective Labeling Aldehydes in DNA

Selective Labeling Aldehydes in DNA

A naphthalimide hydroxylamine probe has been designed and synthesized to selectively label the whole natural aldehydes present in DNAs including 5-formylcytosine, 5-formyluracil, and abasic sites. The fluorescence characteristics of the generated nucleosides have been examined in detail, and the rea...

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Bibliographic Details
Published in:Analytical chemistry (Washington) Vol. 90; no. 24; pp. 14616 - 14621
Main Authors: Liu, Chaoxing, Luo, Xiaomeng, Chen, Yuqi, Wu, Fan, Yang, Wei, Wang, Yafen, Zhang, Xiong, Zou, Guangrong, Zhou, Xiang
Format: Journal Article
Language:English
Published: United States American Chemical Society 18-12-2018
Subjects:
Aldehydes
Analytical chemistry
Chemistry
Deoxyribonucleic acid
DNA
Fluorescence
Hydroxylamine
Labeling
Organic chemistry
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Summary:A naphthalimide hydroxylamine probe has been designed and synthesized to selectively label the whole natural aldehydes present in DNAs including 5-formylcytosine, 5-formyluracil, and abasic sites. The fluorescence characteristics of the generated nucleosides have been examined in detail, and the reaction activities of hydroxylamine, amine groups toward aldehydes in DNA have been discussed with others, which will be a vital reference for designing chemicals for selective labeling of DNAs.
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content type line 23
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.8b04822