Improving Flavonoid Extraction from Ginkgo biloba Leaves by Prefermentation Processing

• Published in: Journal of agricultural and food chemistry Vol. 61; no. 24; pp. 5783 - 5791
• Main Authors: Wang, Jiahong, Cao, Fuliang, Su, Erzheng, Wu, Caie, Zhao, Linguo, Ying, Ruifeng
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 19-06-2013
• Subjects: Antioxidants - analysis; Antioxidants - isolation & purification; Biological and medical sciences; China; culture media; Dietary Supplements - analysis; Feasibility Studies; Fermentation; Flavones - analysis; Flavones - isolation & purification; flavonoids; Food engineering; Food industries; Fundamental and applied biological sciences. Psychology; fungi; Fungi - classification; Fungi - growth & development; Fungi - isolation & purification; Fungi - metabolism; General aspects; General pharmacology; Ginkgo biloba; Ginkgo biloba - chemistry; Ginkgo biloba - growth & development; Ginkgo biloba - microbiology; Hydrolysis; leaves; Lignin - analysis; Lignin - metabolism; Medical sciences; Molecular Typing; Mycological Typing Techniques; nucleotide sequences; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Plant Leaves - chemistry; Plant Leaves - growth & development; Plant Leaves - microbiology; response surface methodology; ribosomal DNA; soil; Soil Microbiology; Species Specificity; temperature; trees; water content; China; solid-state fermentation (SSF); fungus; flavonoids; Ginkgo biloba leaves; response surface methodology (RSM); Methodology; Plant leaf; Fermentation; Flavonoid; Medicinal plant; Ginkgo biloba; Solid state; Polyphenol; Plant part; Gymnospermae; Ginkgoales; Response surface; Spermatophyta; Extraction
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf400712n

This paper presents a prefermentation treatment method involving fungi to improve flavonoid extraction from the leaves of Ginkgo biloba. The fungi employed for this treatment were screened from the soil present under an ancient ginkgo tree. Seventy-six strains belonging to 23 genera were isolated and identified by a molecular identification method employing 18S rDNA sequences. Thirty-three strains grew well using ginkgo leaves as the growth medium. One strain, Gyx086, with higher extracted yield of flavonoids and more similar to the control, was finally selected for prefermentation processing. The major fermentation factors were optimized by response surface methodology. The optimal conditions for the highest total falvonoid yield were 27.8 °C for temperature, 64.2% for moisture content, and 61 h for fermentation time. Under the optimal condition, a actual total flavonoid yield of 27.59 ± 0.52 mg/g dry weight culture sample was obtained, which was about 70% higher than that of unfermented gingko leaf samples.