An Event-Specific DNA Microarray To Identify Genetically Modified Organisms in Processed Foods

• Published in: Journal of agricultural and food chemistry Vol. 58; no. 10; pp. 6018 - 6026
• Main Authors: Kim, Jae-Hwan, Kim, Su-Youn, Lee, Hyungjae, Kim, Young-Rok, Kim, Hae-Yeong
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 26-05-2010
• Subjects: Analytical Methods; Biological and medical sciences; Brassica rapa - genetics; cotton; detection; detection limit; DNA, Plant - analysis; event-specific DNA microarray; Food Analysis - methods; Food industries; Fundamental and applied biological sciences. Psychology; genetic transformation; genetically modified foods; Glycine max - genetics; Gossypium - genetics; microarray technology; molecular sequence data; Oligonucleotide Array Sequence Analysis - methods; Oligonucleotide Probes; plant products; Plants, Genetically Modified - genetics; Polymerase Chain Reaction; processed foods; promoter regions; ribosomal RNA; strain differences; transgenes; transgenic plants; Zea mays - genetics; multiplex PCR; microarray; event-specific; Genetically modified food; Polymerase chain reaction; DNA; Processed food; Molecular biology; Genetically modified organism
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf100351x

We developed an event-specific DNA microarray system to identify 19 genetically modified organisms (GMOs), including two GM soybeans (GTS-40-3-2 and A2704-12), thirteen GM maizes (Bt176, Bt11, MON810, MON863, NK603, GA21, T25, TC1507, Bt10, DAS59122-7, TC6275, MIR604, and LY038), three GM canolas (GT73, MS8×RF3, and T45), and one GM cotton (LLcotton25). The microarray included 27 oligonucleotide probes optimized to identify endogenous reference targets, event-specific targets, screening targets (35S promoter and nos terminator), and an internal target (18S rRNA gene). Thirty-seven maize-containing food products purchased from South Korean and US markets were tested for the presence of GM maize using this microarray system. Thirteen GM maize events were simultaneously detected using multiplex PCR coupled with microarray on a single chip, at a limit of detection of approximately 0.5%. Using the system described here, we detected GM maize in 11 of the 37 food samples tested. These results suggest that an event-specific DNA microarray system can reliably detect GMOs in processed foods.