SPME Applied to the Study of Volatile Organic Compounds Emitted by Three Species of Eucalyptus in Situ

• Published in: Journal of agricultural and food chemistry Vol. 50; no. 25; pp. 7199 - 7205
• Main Authors: Zini, Cláudia A, Augusto, Fabio, Christensen, Eva, Caramão, Elina B, Pawliszyn, Janusz
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 04-12-2002
• Subjects: Biological and medical sciences; Chemical constitution; Eucalyptus; Fundamental and applied biological sciences. Psychology; Plant physiology and development; Gas emission; Biochemistry; Gas analysis; Eucalyptus; Dicotyledones; Angiospermae; Solid-phase micro extraction (SPME); Eucalyptus citriodora; Eucalyptus dunnil; Eucalyptus saligna; Myrtaceae; Hardwood forest tree; Chemical composition; Continuous measurement; Species; Method study; Analytical chemistry; Volatile compound; Volatile organic compound; Woody plant; Experimental study; Experimental protocol; Measurement in situ; Solid phase microextraction; Organic chemistry; Biogeochemistry; Analysis method; Forestry; Plant origin; Spermatophyta; sampling chamber; blogenic volatile organic compounds (BVOC); rose oxide; (E)β-oclmene
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf025666m

Headspace solid-phase microextraction coupled to gas chromatography/ion trap mass spectrometry−65 μm polydimethylsiloxane/divinylbenzene (PDMS/DVB) was used to identify and monitor the emission patterns of biogenic volatile organic compounds from leaves of Eucalyptus dunnii, Eucalyptus saligna, and Eucalyptus citriodora in situ. Short extractions (1 min) were performed every 30 min for periods of 8−10 h during 24 days taking advantage of the high capacity of this porous polymer coating. Forty-two compounds were detected and 20 identified in the headspace of E. saligna leaves, and 19 of 27 compounds were identified in the headspace of E. dunnii leaves. The emission pattern of (E)-β-ocimene and rose oxide suggests that they may play a bioactive role in Eucalyptus. Keywords: Solid-phase micro extraction (SPME); Eucalyptus citriodora; Eucalyptus dunnii; Eucalyptus saligna, sampling chamber; biogenic volatile organic compounds (BVOC); rose oxide; (E)-β-ocimene