A Strategy for the Sequential Patterning of Proteins: Catalytically Active Multiprotein Nanofabrication

We describe a new and simple method for immobilizing enzymes that will allow the preparation of thin films containing several different catalytically active enzymes. We use this method to immobilize acetylcholine esterase, then we show that its catalytic activity is retained after immobilizing a dif...

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Bibliographic Details
Published in:	Nano letters Vol. 3; no. 6; pp. 691 - 694
Main Authors:	Jang, Chang-Hyun, Stevens, Benjamin D, Phillips, Ryan, Calter, Michael A, Ducker, William A
Format:	Journal Article
Language:	English
Published:	Washington, DC American Chemical Society 01-06-2003
Subjects:	Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology Immobilization of enzymes and other molecules Immobilization techniques Methods. Procedures. Technologies
Online Access:	Get full text
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Summary:	We describe a new and simple method for immobilizing enzymes that will allow the preparation of thin films containing several different catalytically active enzymes. We use this method to immobilize acetylcholine esterase, then we show that its catalytic activity is retained after immobilizing a different protein on the same film.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	1530-6984 1530-6992
DOI:	10.1021/nl0259636