Effect of Osteopontin, Chondroitin Sulfates (B, C), and Human Serum Albumin on the Crystallization Behavior of Hydroxyapatite in Agarose and Silica Hydrogels

• Published in: Crystal growth & design Vol. 8; no. 4; pp. 1335 - 1339
• Main Authors: Jiménez-Corona, Azucena E, Pérez-Torres, Armando, Mas-Oliva, Jaime, Moreno, Abel
• Format: Journal Article
• Language: English
• Published: Washington,DC American Chemical Society 01-04-2008
• Subjects: Condensed matter: structure, mechanical and thermal properties; Cross-disciplinary physics: materials science; rheology; Equations of state, phase equilibria, and phase transitions; Exact sciences and technology; Growth from solutions; Materials science; Methods of crystal growth; physics of crystal growth; Physics; Solid-solid transitions; Specific phase transitions; Crystal growth; Crystallization; Osteopontin; Hydroxyapatite; Biological macromolecule; XRD; Sulfates; Silica; Agarose; Albumins; Chondroitin sulfate C; Experimental design; Chondroitin sulfate B; Crystal growth from solutions; Crystal nucleation; Hydrogel
• ISSN: 1528-7483; 1528-7505
• DOI: 10.1021/cg7011414

This contribution describes the nucleation and crystal growth behavior of hydroxyapatite (HAP) crystals grown in agarose and silica hydrogels as well as their characterization via powder X-ray diffraction. The effect of several biological macromolecules such as osteopontin, chondroitin sulfates B and C, and human serum albumin (HSA) on crystal growth behavior of HAP is evaluated. From our results, osteopontin and chondroitin sulfates B and C inhibit the HAP crystal growth in agarose hydrogels. Nevertheless, employing silica hydrogels, only osteopontin inhibits the crystal growth of HAP. On the other hand, HSA does not show any effect on the formation of HAP crystals in both types of hydrogels.