Stage-Specific Fatty Acid Fluxes Play a Regulatory Role in Glycerolipid Metabolism during Seed Development in Jatropha curcas L

• Published in: Journal of agricultural and food chemistry Vol. 63; no. 50; pp. 10811 - 10821
• Main Authors: Chaitanya, Bharatula Sri Krishna, Kumar, Sumit, Kaki, Shiva Shanker, Balakrishna, Marrapu, Karuna, Mallampalli Sri Lakshmi, Prasad, Rachapudi Badari Narayana, Sastry, Pidaparty Seshadri, Reddy, Attipalli Ramachandra
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 23-12-2015
• Subjects: Diglycerides - analysis; Endosperm - chemistry; Fatty Acids - analysis; Gene Expression; Glycerophospholipids - metabolism; Jatropha - growth & development; Oleic Acid - analysis; Palmitic Acid - analysis; Phosphatidylcholines - biosynthesis; Phosphatidylcholines - chemistry; Phosphatidylcholines - metabolism; Phospholipids - analysis; Seeds - chemistry; Seeds - genetics; Seeds - growth & development; Time Factors; Triglycerides - analysis; 18:1-rich TAG; acyl-edited DAG; Jatropha curcas; phosphatidylcholine
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/acs.jafc.5b04824

The present study describes the changes in lipid profile as well as fatty acid fluxes during seed development in Jatropha curcas L. Endosperm from 34, 37, and 40 days after anthesis (DAA), incubated with [14C]­acetate, showed significant synthesis of phosphatidylcholine (PC) at seed maturation. The fatty acid methyl ester profile showed PC from 34 DAA was rich in palmitic acid (16:0), whereas PC from 37 and 40 DAA was rich in oleic acid (18:1n-9). Molecular species analysis of diacylglycerol (DAG) indicated DAG (16:0/18:2n-6) was in abundance at 34 DAA, whereas DAG (18:1n-9/18:2n-6) was significantly high at 40 DAA. Triacylglycerol (TAG) analysis revealed TAG (16:0/18:2n-6/16:0) was abundant at 34 DAA, whereas TAG (18:1n-9/18:2n-6/18:1n-9) formed the majority at 40 DAA. Expression of two types of diacylglycerol acyltransferases varied with seed maturation. These data demonstrate stage-specific distinct pools of PC and DAG synthesis during storage TAG accumulation in Jatropha seed.