Orientation and Function of a Membrane-Bound Enzyme Monitored by Electrochemical Surface-Enhanced Infrared Absorption Spectroscopy

Orientation and Function of a Membrane-Bound Enzyme Monitored by Electrochemical Surface-Enhanced Infrared Absorption Spectroscopy

Electrochemical SEIRA is the method of choice for characterizing a lipid-bilayer-anchored, membrane-bound hydrogenase immobilized on a gold electrode. This setup allows the study of the enzyme under conditions mimicking its natural environment. A single experiment provides all of the crucial spectro...

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Bibliographic Details
Published in:The journal of physical chemistry letters Vol. 4; no. 17; pp. 2794 - 2798
Main Authors: Gutiérrez-Sanz, Oscar, Marques, Marta, Pereira, Inês A. C, De Lacey, Antonio L, Lubitz, Wolfgang, Rüdiger, Olaf
Format: Journal Article
Language:English
Published: American Chemical Society 05-09-2013
Subjects:
Biophysical Chemistry and Biomolecules
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Summary:Electrochemical SEIRA is the method of choice for characterizing a lipid-bilayer-anchored, membrane-bound hydrogenase immobilized on a gold electrode. This setup allows the study of the enzyme under conditions mimicking its natural environment. A single experiment provides all of the crucial spectroscopic information relating to the protein orientation, active site of the protein, and the lipid bilayer and also direct electrochemical determination of the catalytic H2 oxidation by the enzyme.
ISSN:1948-7185
1948-7185
DOI:10.1021/jz4013678