Prostaglandin-induced iris color darkening. An experimental model

• Published in: Archives of ophthalmology (1960) Vol. 116; no. 8; p. 1065
• Main Authors: Zhan, G L, Toris, C B, Camras, C B, Wang, Y L, Bito, L Z
• Format: Journal Article
• Language: English
• Published: United States 01-08-1998
• Subjects: Administration, Topical; Animals; Eye Color - drug effects; Eye Color - physiology; Female; Ganglia, Sympathetic - physiology; Ganglionectomy; Hyperpigmentation - chemically induced; Hyperpigmentation - physiopathology; Iris - drug effects; Iris - innervation; Latanoprost; Models, Biological; Ophthalmic Solutions; Photography; Prostaglandins F, Synthetic - administration & dosage; Prostaglandins F, Synthetic - pharmacology; Rabbits
• ISSN: 0003-9950
• DOI: 10.1001/archopht.116.8.1065

To determine the role of sympathetic innervation and the effect of topical prostaglandin therapy on iris color in pigmented rabbits.
Twelve Dutch-belted rabbits underwent unilateral superior cervical ganglionectomy (SCGx) at age 1 to 3 months. A second group of 11 rabbits underwent bilateral SCGx at age 1 month and were treated once or twice daily for 6 to 9 months with 1 drop (about 20 microL) of latanoprost, 0.005%, to one eye and its vehicle to the contralateral eye. Standardized color photographs of the iris of each eye were taken at 1- to 2-month intervals for 6 to 10 months and evaluated by 4 to 6 observers in a masked fashion.
At 8 to 10 months after unilateral SCGx, 11 of 12 rabbits showed definite heterochromia, with the lighter-colored iris on the SCGx side. Of the 11 rabbits that underwent bilateral SCGx and unilateral latanoprost treatment, 9 showed heterochromia at 6 to 9 months, with the darker-colored iris on the latanoprost-treated side.
These results demonstrate that sympathetic innervation is required for age-related, physiologic darkening of iris color in rabbits, that prostaglandins may compensate for sympathetic denervation to produce darkening in SCGx eyes, and that this model may be useful to study prostaglandin-induced iris color change.