Molecular Concentration Determination Using Long-Interval Chemical Exchange Inversion Transfer (CEIT) NMR Spectroscopy

• Published in: The journal of physical chemistry letters Vol. 12; no. 35; pp. 8652 - 8657
• Main Authors: Jiang, Weiping, Guo, Qianni, Luo, Qing, Zhang, Xiaoxiao, Yuan, Yaping, Li, Haidong, Zhou, Xin
• Format: Journal Article
• Language: English
• Published: American Chemical Society 09-09-2021
• Subjects: Physical Insights into Chemistry, Catalysis, and Interfaces
• ISSN: 1948-7185; 1948-7185
• DOI: 10.1021/acs.jpclett.1c02239

Functionalized hyperpolarized xenon “cage” molecules have often been used for ultrasensitive detection of biomolecules and microenvironment properties. However, the rapid and accurate measurement of molecule concentration is still a challenge. Here, we report a molecule concentration measurement method using long-interval chemical exchange inversion transfer (CEIT) NMR spectroscopy. The molecule concentration can be quantitatively measured with only 2 scans, which shortens the acquisition time by about 10 times compared to conventional Hyper-CEST (chemical exchange saturation transfer) z-spectrum method. Moreover, we found that the accuracy of concentration determination would be the best when the CEIT effect is 1–1/e or close to it, and a relative deviation of CrA-(COOH)6 less than ±1% has been achieved by only a one-step optimization of the number of cycles. The proposed method enables efficient and accurate determination of molecule concentration, which provides a potential way for rapid quantitative molecular imaging applications.