Quick Refolding of High-Concentration Proteins via Microchannel Dialysis

Quick Refolding of High-Concentration Proteins via Microchannel Dialysis

The industrial production of active proteins from Escherichia coli necessitates the refolding of high concentrations of protein over a short period of time. However, it is difficult to simultaneously achieve high concentration and short operation time. In the present study, by designing microchannel...

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Bibliographic Details
Published in:Industrial & engineering chemistry research Vol. 60; no. 28; pp. 10076 - 10082
Main Authors: Kato, Aoi, Ohashi, Hidenori
Format: Journal Article
Language:English
Published: American Chemical Society 21-07-2021
Subjects:
Bioengineering
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Summary:The industrial production of active proteins from Escherichia coli necessitates the refolding of high concentrations of protein over a short period of time. However, it is difficult to simultaneously achieve high concentration and short operation time. In the present study, by designing microchannels with dialysis membranes by a chemical engineering way, the surface area was enlarged for quickly removing the protein denaturant and high-concentration protein refolding was achieved in a short time; high concentrations of active carbonic anhydrase8 times more concentrated than achieved using dilution methodwere refolded in 20 min, which is orders of magnitude faster than the conventional dialysis method.
ISSN:0888-5885
1520-5045
DOI:10.1021/acs.iecr.1c00410