Optimization of Culture Conditions for Maximal Lovastatin Production by Aspergillus terreus (KM017963) under Solid State Fermentation

• Published in: Hayati : journal of biosciences Vol. 22; no. 4; pp. 174 - 180
• Main Authors: Kamath, Praveen Vadakke, Dwarakanath, Bhargavi Santebennur, Chaudhary, Avinash, Janakiraman, Savitha
• Format: Journal Article
• Language: English
• Published: Bogor Bogor Agricultural University (IPB), Department of Biology 01-10-2015; Bogor Agricultural University
• Subjects: Agricultural production; Amino acids; Aspergillus terreus; Averrhoa; Biosynthesis; Calcium chloride; Carbon; Carbon sources; Cellulose; Cholesterol; Chromatography; Conflicts of interest; Culture; Dextrin; Dietary supplements; Fermentation; Hydrocarbons; Inoculum; Iron sulfates; Lovastatin; Metal ions; Metals; Microorganisms; Nitrogen sources; Nutrients; Optimization; Oryza; Oryza longistaminata; pH effects; Physical factors; Pollutants; Salts; Seeds; Sodium chloride; Solid state; Solid state fermentation; Spores; Sugarcane; Surfactants; Temperature; Wheat; Wheat bran; United States > US
• ISSN: 1978-3019; 2086-4094
• DOI: 10.1016/j.hjb.2015.11.001

Effects of various culture conditions on lovastatin production were investigated in Aspergillus terreus (KM017963) grown under solid state fermentation with wheat bran. Lovastatin production was influenced by various physical factors such as pH, temperature, and nutritional factors such as carbon, nitrogen, metal ions/salts etc. Our study established that an initial pH of 6.0, growth temperature of 28 °C–30 °C, inoculum size of 108 spores/mL as the optimal physiological culture conditions for maximal production of lovastatin by A. terreus (KM017963). The carbon sources, glucose or dextrin when supplemented at 3% (w/w) enhanced lovastatin production by five fold when supplemented as individual component in wheat bran. Addition of metal salts such as CuSO4 (8%), FeSO4 (8%), CaCl2 (10%), NaCl (6%) and MgSO4 (6%) enhanced the production by five fold. Supplementation with nitrogen sources, amino acids, hydrocarbons, surfactants and amino acids did not have any profound effect on lovastatin production.