Topography-driven bionano-interactions on colloidal silica nanoparticles

• Published in: ACS applied materials & interfaces Vol. 6; no. 5; pp. 3437 - 3447
• Main Authors: Paula, Amauri J, Silveira, Camila P, Martinez, Diego Stéfani T, Souza Filho, Antonio G, Romero, Fabian V, Fonseca, Leandro C, Tasic, Ljubica, Alves, Oswaldo L, Durán, Nelson
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 12-03-2014
• Subjects: Adsorption; Animals; Cattle; Colloids; Humans; Nanoparticles - chemistry; Plasma - chemistry; Serum Albumin, Bovine - chemistry; Silicon Dioxide - chemistry; Surface Properties; colloidal nanoparticles; alginate; mesoporous silica; human blood plasma; biomolecules interaction; protein corona; bovine serum albumin
• ISSN: 1944-8244; 1944-8252
• DOI: 10.1021/am405594q

We report here that the surface topography of colloidal mesoporous silica nanoparticles (MSNs) plays a key role on their bionano-interactions by driving the adsorption of biomolecules on the nanoparticle through a matching mechanism between the surface cavities characteristics and the biomolecules stereochemistry. This conclusion was drawn by analyzing the biophysicochemical properties of colloidal MSNs in the presence of single biomolecules, such as alginate or bovine serum albumin (BSA), as well as dispersed in a complex biofluid, such as human blood plasma. When dispersed in phosphate buffered saline media containing alginate or BSA, monodisperse spherical MSNs interact with linear biopolymers such as alginate and with a globular protein such as bovine serum albumin (BSA) independently of the surface charge sign (i.e. positive or negative), thus leading to a decrease in the surface energy and to the colloidal stabilization of these nanoparticles. In contrast, silica nanoparticles with irregular surface topographies are not colloidally stabilized in the presence of alginate but they are electrosterically stabilized by BSA through a sorption mechanism that implies reversible conformation changes of the protein, as evidenced by circular dichroism (CD). The match between the biomolecule size and stereochemistry with the nanoparticle surface cavities characteristics reflects on the nanoparticle surface area that is accessible for each biomolecule to interact and stabilize any non-rigid nanoparticles. On the other hand, in contact with variety of biomolecules such as those present in blood plasma (55%), MSNs are colloidally stabilized regardless of the topography and surface charge, although the identity of the protein corona responsible for this stabilization is influenced by the surface topography and surface charge. Therefore, the biofluid in which nanoparticles are introduced plays an important role on their physicochemical behavior synergistically with their inherent characteristics (e.g., surface topography).