Proteomic Analysis of the Outer Membrane of Anabaena sp. Strain PCC 7120

Anabaena is a model to analyze the evolutionary development of plastids, cell differentiation, and the regulation of nitrogen fixation. Thereby, the outer membrane proteome is the place of sensing environmental differences and during plastid development, systems for intracellular communication had t...

Full description

Saved in:

Bibliographic Details
Published in:	Journal of proteome research Vol. 4; no. 4; pp. 1330 - 1338
Main Authors:	Moslavac, Suncana, Bredemeier, Rolf, Mirus, Oliver, Granvogl, Bernhard, Eichacker, Lutz A, Schleiff, Enrico
Format:	Journal Article
Language:	English
Published:	United States American Chemical Society 01-07-2005
Subjects:	Anabaena - cytology Bacterial Outer Membrane Proteins - analysis Bacterial Outer Membrane Proteins - isolation & purification Cell Fractionation - methods Cell Membrane - chemistry Computational Biology Molecular Sequence Data Proteome - analysis
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Anabaena is a model to analyze the evolutionary development of plastids, cell differentiation, and the regulation of nitrogen fixation. Thereby, the outer membrane proteome is the place of sensing environmental differences and during plastid development, systems for intracellular communication had to be added to the proteome of this membrane. We present a protocol for the isolation of the outer membrane from Anabaena and the analysis of the proteome using different tools. 55 Proteins were identified. Keywords: proteome analysis • β-barrel analysis • Anabaena • outer membrane
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1535-3893 1535-3907
DOI:	10.1021/pr050044c