Loss of Allostery and Coenzyme B12 Delivery by a Pathogenic Mutation in Adenosyltransferase

• Published in: Biochemistry (Easton) Vol. 50; no. 25; pp. 5790 - 5798
• Main Authors: Lofgren, Michael, Banerjee, Ruma
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 28-06-2011
• Subjects: Allosteric Regulation - genetics; Amino Acid Metabolism, Inborn Errors - enzymology; Amino Acid Metabolism, Inborn Errors - genetics; Amino Acid Sequence; Cobamides - deficiency; Cobamides - genetics; Gene Deletion; Humans; Methionine Adenosyltransferase - biosynthesis; Methionine Adenosyltransferase - deficiency; Methionine Adenosyltransferase - genetics; Methylobacterium extorquens - enzymology; Methylobacterium extorquens - genetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Transport - genetics
• ISSN: 0006-2960; 1520-4995
• DOI: 10.1021/bi2006306

ATP-dependent cob(I)alamin adenosyltransferase (ATR) is a bifunctional protein: an enzyme that catalyzes the adenosylation of cob(I)alamin and an escort that delivers the product, adenosylcobalamin (AdoCbl or coenzyme B12), to methylmalonyl-CoA mutase (MCM), resulting in holoenzyme formation. Failure to assemble holo-MCM leads to methylmalonic aciduria. We have previously demonstrated that only 2 equiv of AdoCbl bind per homotrimer of ATR and that binding of ATP to the vacant active site triggers ejection of 1 equiv of AdoCbl from an adjacent site. In this study, we have mimicked in the Methylobacterium extorquens ATR, a C-terminal truncation mutation, D180X, described in a patient with methylmalonic aciduria, and characterized the associated biochemical penalties. We demonstrate that while k cat and K M Cob(I) for D180X ATR are only modestly decreased (by 3- and 2-fold, respectively), affinity for the product, AdoCbl, is significantly diminished (400-fold), and the negative cooperativity associated with its binding is lost. We also demonstrate that the D180X mutation corrupts ATP-dependent cofactor ejection, which leads to transfer of AdoCbl from wild-type ATR to MCM. These results suggest that the pathogenicity of the corresponding human truncation mutant results from its inability to sequester AdoCbl for direct transfer to MCM. Instead, cofactor release into solution is predicted to reduce the capacity for holo-MCM formation, leading to disease.