Simultaneous analysis of vitamins A and E in rodent feed by high-pressure liquid chromatography

• Published in: Journal of agricultural and food chemistry Vol. 39; no. 2; pp. 296 - 299
• Main Authors: Rushing, Larry G, Cooper, Willie M, Thompson, Harold C
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01-02-1991
• Subjects: ALIMENT POUR ANIMAUX; ALIMENTOS; Biological and medical sciences; EVALUACION; EVALUATION; Feed and pet food industries; Food industries; Fundamental and applied biological sciences. Psychology; INVESTIGACION; OXIDACION; OXYDATION; PIENSOS; PRODUIT ALIMENTAIRE; RECHERCHE; RETINOL; ROEDORES; RONGEUR; VITAMINA E; VITAMINE E; Assay; Vertebrata; Mammalia; Quality control; Analytical method; Rodentia; Vitamin; HPLC chromatography; Chemical composition; Feed
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf00002a015

A method is presented for the coextraction and simultaneous analysis of vitamins A and E from three rodent feeds (NIH-31, Masoro, and Emory Morse) formulations. The method consists of extraction of the vitamins for 16 h with ethanolic KOH containing ascorbic acid followed by partitioning into hexane and subsequent analysis by normal phase HPLC using hexane/2-propanol/glacial acetic acid (990/10/0.2) mobile phase, a 5-micrometer LC-CN column, and detection at 296 nm. Recoveries of vitamin A from NIH-31, Masoro, and Emory Morse feeds spiked at 50 micrograms/g were 95.5, 74.6, and 78.2%, respectively, while those for vitamin E spiked at 250 micrograms/g were 103, 103, and 85.2%, respectively. Ancillary data are also presented which demonstrate the stability of vitamin A alcohol and vitamin E in ethanolic KOH for as long as 21 h. Additional data are presented detailing results of analyses of five lots of NIH-31 and Masoro feeds and seven lots of Emory Morse feed as received from the supplier