Effects of Amino Acid Additives on Protein Stability during Electrothermal Supercharging in ESI-MS

• Published in: Journal of the American Society for Mass Spectrometry Vol. 35; no. 1; pp. 151 - 157
• Main Authors: Javanshad, Roshan, Panth, Rajendra, Venter, Andre R
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 03-01-2024
• Subjects: ammonium bicarbonate; ESI-MS; electrothermal supercharging; protein supercharging
• ISSN: 1044-0305; 1879-1123
• DOI: 10.1021/jasms.3c00377

The surprising formation of highly charged protein ions from aqueous ammonium bicarbonate solution is a fascinating phenomenon referred to as electrothermal supercharging (ETS). Although the precise mechanism involved is not clearly understood, previous studies predominantly suggest that ETS is due to native protein destabilization in the presence of bicarbonate anion inside the electrospray ionization droplets under high temperatures and spray voltages. To evaluate existing hypotheses surrounding the underlying mechanism of ETS, the effects of several additives on protein charging under ETS conditions were investigated. The changes in the protein charge state distributions were compared by measuring the ratios between the intensities of highest intensity charge states of native and unfolded protein envelopes and shifts in the lowest and highest observed charge states. This study demonstrated that source temperature plays a more important role in ETS compared to spray voltage, especially when using a nebulized microelectrospray ionization source. Moreover, the effect of amino acids on ETS were generally in good agreement with the extensive literature available on the stabilization or destabilization of proteins by these additives in bulk solution. Among the natural amino acids, protein supercharging was significantly reduced by proline and glycine; however, imidazole provided the highest degree of noncovalent complex stabilization against ETS, outperforming the amino acids. Overall, our study shows that the simple addition of stabilizing reagents such as proline and imidazole can reduce the extent of apparent protein unfolding and supercharging in ammonium bicarbonate solution and provide evidence against the roles of charge depletion and thermal unfolding during ETS.