Storage and metabolism of poly(adenylic acid)-mRNA in germinating cotton seeds

• Published in: Biochemistry (Easton) Vol. 14; no. 20; pp. 4375 - 4379
• Main Authors: Hammett, J. R, Katterman, F. R
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 01-10-1975
• Subjects: Base Sequence; Cell Nucleus - metabolism; Chromatography, Affinity; Deoxyadenosines - pharmacology; Gossypium; Plant Proteins - biosynthesis; Poly A - isolation & purification; Poly A - metabolism; Polyribosomes - metabolism; Protein Biosynthesis - drug effects; RNA, Messenger - isolation & purification; RNA, Messenger - metabolism; Seeds - metabolism
• ISSN: 0006-2960; 1520-4995
• DOI: 10.1021/bi00691a005

When the RNA from both the nucleus and polysomes of germinated cotton seeds were fractionated on poly(U)-cellulose columns it was found that both contained poly(A)-RNA. In ungerminated seeds most of the poly(A)-RNA is located in the nucleus of the cell. 3'-Deoxyadenosine was found to inhibit the synthesis of both nuclear and polysomal poly(A) by 75% in germinated seeds, but had little or no effect on protein synthesis during the first 6 hr of germination. After this time 3'-deoxyadenosine had a pronounced inhibitory effect on protein synthesis presumably due to the inhibition of poly(A) addition to newly synthesized mRNA.