Sensitive Fluorescent Sensor for Hydrogen Sulfide in Rat Brain Microdialysis via CsPbBr3 Quantum Dots

• Published in: Analytical chemistry (Washington) Vol. 91; no. 24; pp. 15915 - 15921
• Main Authors: Chen, Chaoqun, Cai, Qing, Luo, Fang, Dong, Nuo, Guo, Longhua, Qiu, Bin, Lin, Zhenyu
• Format: Journal Article
• Language: English
• Published: Washington American Chemical Society 17-12-2019
• Subjects: Analytical chemistry; Aqueous solutions; Brain; Chemistry; Fluorescence; Hydrogen; Hydrogen sulfide; Microdialysis; n-Hexane; Perovskites; Phosphoric acid; Pollutants; Quantum dots; Quenching; Selectivity
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/acs.analchem.9b04387

The instability and insolubility of perovskite quantum dots in aqueous solution prohibit applications in polar solvents. As a highly toxic gas pollutant and also an endogenous gaseous signaling molecule existing in a variety of physiological processes, hydrogen sulfide (H2S), with high selectivity and high specificity, detection is of great significance. In this study, a simple device has been designed to separate H2S from aqueous solution and CsPbBr3 quantum dots (CsPbBr3 QDs) have been used as the detection probe to develop a novel fluorescent sensor for rapid H2S detection. The addition of hydrogen sulfide to the phosphoric acid solution results in the escape of H2S from the aqueous sample and hence it passing into the n-hexane solution containing CsPbBr3 QDs, resulting in the quenching of the fluorescence of CsPbBr3 QDs. The fluorescence intensity of the system has a linear relationship with the concentration of H2S in the range of 0–100 μM with the detection limit of 0.18 μM. The proposed system has been applied to detection of H2S in rat brain microdialysate with satisfying results. The potential mechanism regarding the quenching of fluorescence from CsPbBr3 QDs by H2S has been studied as well.