TRIM14对宫颈癌细胞增殖、迁移和侵袭及上皮—间充质转化的影响及其作用机制
R737.33; 目的:探讨三结构域蛋白14(TRIM14)对宫颈癌细胞增殖、迁移、侵袭及上皮—间充质转化(EMT)的影响及相关作用机制.方法:收集40例宫颈癌患者的宫颈癌组织及癌旁正常宫颈组织标本,采用实时荧光定量PCR(RT-qPCR)法检测TRIM14基因表达.体外培养宫颈癌HeLa细胞,将TRIM14小干扰RNA(si-TRIM14)转染至HeLa细胞.采用CCK-8法检测细胞增殖,划痕实验检测细胞迁移,Transwell小室法检测细胞侵袭,Western blotting法检测EMT相关蛋白和单磷酸腺苷蛋白激酶(AMPK)/雷帕霉素靶蛋白(mTOR)信号通路相关蛋白表达.结果:宫颈癌...
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| Published in: | 广西医科大学学报 Vol. 40; no. 1; pp. 101 - 106 |
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| Main Authors: | , , |
| Format: | Journal Article |
| Language: | Chinese |
| Published: |
河北北方学院附属第一医院放疗科,张家口 075000
2023
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| Abstract | R737.33; 目的:探讨三结构域蛋白14(TRIM14)对宫颈癌细胞增殖、迁移、侵袭及上皮—间充质转化(EMT)的影响及相关作用机制.方法:收集40例宫颈癌患者的宫颈癌组织及癌旁正常宫颈组织标本,采用实时荧光定量PCR(RT-qPCR)法检测TRIM14基因表达.体外培养宫颈癌HeLa细胞,将TRIM14小干扰RNA(si-TRIM14)转染至HeLa细胞.采用CCK-8法检测细胞增殖,划痕实验检测细胞迁移,Transwell小室法检测细胞侵袭,Western blotting法检测EMT相关蛋白和单磷酸腺苷蛋白激酶(AMPK)/雷帕霉素靶蛋白(mTOR)信号通路相关蛋白表达.结果:宫颈癌组织中TRIM14 mRNA表达水平显著高于癌旁正常宫颈组织(P<0.05).与si-NC组比较,si-TRIM14组细胞增殖活性、细胞迁移率、侵袭细胞数目均降低,E-cadherin蛋白表达量升高,N-cadherin、Vimentin、Snail1蛋白表达量均降低(P<0.05).si-TRIM14组细胞中p-AMPK/AMPK比值高于si-NC组,p-mTOR/mTOR比值低于si-NC组(P<0.05).结论:TRIM14在宫颈癌组织中高表达,干扰TRIM14表达可通过调控AMPK/mTOR信号通路抑制EMT,进而抑制宫颈癌细胞增殖、迁移和侵袭. |
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| AbstractList | R737.33; 目的:探讨三结构域蛋白14(TRIM14)对宫颈癌细胞增殖、迁移、侵袭及上皮—间充质转化(EMT)的影响及相关作用机制.方法:收集40例宫颈癌患者的宫颈癌组织及癌旁正常宫颈组织标本,采用实时荧光定量PCR(RT-qPCR)法检测TRIM14基因表达.体外培养宫颈癌HeLa细胞,将TRIM14小干扰RNA(si-TRIM14)转染至HeLa细胞.采用CCK-8法检测细胞增殖,划痕实验检测细胞迁移,Transwell小室法检测细胞侵袭,Western blotting法检测EMT相关蛋白和单磷酸腺苷蛋白激酶(AMPK)/雷帕霉素靶蛋白(mTOR)信号通路相关蛋白表达.结果:宫颈癌组织中TRIM14 mRNA表达水平显著高于癌旁正常宫颈组织(P<0.05).与si-NC组比较,si-TRIM14组细胞增殖活性、细胞迁移率、侵袭细胞数目均降低,E-cadherin蛋白表达量升高,N-cadherin、Vimentin、Snail1蛋白表达量均降低(P<0.05).si-TRIM14组细胞中p-AMPK/AMPK比值高于si-NC组,p-mTOR/mTOR比值低于si-NC组(P<0.05).结论:TRIM14在宫颈癌组织中高表达,干扰TRIM14表达可通过调控AMPK/mTOR信号通路抑制EMT,进而抑制宫颈癌细胞增殖、迁移和侵袭. |
| Author | 张志林 张贤雨 马欢 |
| AuthorAffiliation | 河北北方学院附属第一医院放疗科,张家口 075000 |
| AuthorAffiliation_xml | – name: 河北北方学院附属第一医院放疗科,张家口 075000 |
| Author_FL | Zhang Xianyu Ma Huan Zhang Zhilin |
| Author_FL_xml | – sequence: 1 fullname: Zhang Xianyu – sequence: 2 fullname: Ma Huan – sequence: 3 fullname: Zhang Zhilin |
| Author_xml | – sequence: 1 fullname: 张贤雨 – sequence: 2 fullname: 马欢 – sequence: 3 fullname: 张志林 |
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| DocumentTitle_FL | Effect of TRIM14 on proliferation, migration, invasion and epithelial-to-mesenchymal transi-tion of cervical cancer cells and its mechanism |
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| Keywords | 侵袭 宫颈癌 三结构域蛋白14 迁移 上皮—间充质转化 |
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| Title | TRIM14对宫颈癌细胞增殖、迁移和侵袭及上皮—间充质转化的影响及其作用机制 |
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