Organism-Level Tumor Models in Zebrafish Danio rerio

• Published in: Actanaturae Vol. 10; no. 2; pp. 24 - 29
• Main Authors: Mizgirev, I. V., Safina, D. R., Demidyuk, I. V., Kostrov, S. V.
• Format: Journal Article
• Language: English
• Published: A.I. Gordeyev 01-01-2018
• ISSN: 2075-8251

Development and implementation of adequate organism-level models is one of the
key elements in biomedical research that focuses on experimental oncology. Over
the last decade, studies using Zebrafish (
Danio rerio
) have
gained in popularity in this area of research. This review describes the
various approaches that have been used in developing highly effective models
for oncological (clinical term, better cancer or tumor) studies based on
D. rerio
. Priority is given to transplantation models of
cancer and their application to optically transparent
D. rerio
lines, including clonal ones, and utilization tumors of various
origins bearing fluorescent labels. The combination of tumor transplantation at
organism-level models in transparent clonal
D. rerio
lines
with fluorescent microscopy, FACS-fractionation of tumor cell subsets, and
transcription analysis can result in one of the most promising research
approaches in providing new information on tumor formation and growth.