The molecular chaperone Brichos breaks the catalytic cycle that generates toxic Aβ oligomers

• Published in: Nature structural & molecular biology Vol. 22; no. 3; pp. 207 - 213
• Main Authors: Cohen, Samuel I. A., Arosio, Paolo, Presto, Jenny, Kurudenkandy, Firoz Roshan, Biverstal, Henrik, Dolfe, Lisa, Dunning, Christopher, Yang, Xiaoting, Frohm, Birgitta, Vendruscolo, Michele, Johansson, Jan, Dobson, Christopher M., Fisahn, André, Knowles, Tuomas P. J., Linse, Sara
• Format: Journal Article
• Language: English
• Published: 16-02-2015
• ISSN: 1545-9993; 1545-9985
• DOI: 10.1038/nsmb.2971

Alzheimer’s disease is an increasingly prevalent neurodegenerative disorder whose pathogenesis has been associated with aggregation of the amyloid-β peptide (Aβ42). Recent studies have revealed that once Aβ42 fibrils are generated, their surfaces strongly catalyse the formation of neurotoxic oligomers. Here we show that a molecular chaperone, a Brichos domain, can specifically inhibit this catalytic cycle and limit Aβ42 toxicity. We demonstrate
in vitro
that Brichos achieves this inhibition by binding to the surfaces of fibrils, thereby redirecting the aggregation reaction to a pathway that involves minimal formation of toxic oligomeric intermediates. We verify that this mechanism occurs in living brain tissue by means of cytotoxicity and electrophysiology experiments. These results reveal that molecular chaperones can help maintain protein homeostasis by selectively suppressing critical microscopic steps within the complex reaction pathways responsible for the toxic effects of protein misfolding and aggregation.