Combination of Radiation and Burn Injury Alters FDG Uptake in Mice

• Published in: Journal of burn care & research Vol. 33; no. 6; pp. 723 - 730
• Main Authors: Carter, Edward A., Winter, David, Tolman, Crystal, Paul, Kasie, Hamrahi, Victoria, Tompkins, Ronald, Fischman, Alan J.
• Format: Journal Article
• Language: English
• Published: 01-01-2012
• ISSN: 1559-047X; 1559-0488
• DOI: 10.1097/BCR.0b013e31825d678f

Radiation exposure and burn injury have both been shown to alter glucose utilization in vivo. The present study was designed to study the effect of burn injury combined with radiation exposure, on glucose metabolism in mice using [
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F] Fluorodeoxyglucose (
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FDG). Groups of male mice weighing approximately 30g were studied. Group 1 was irradiated with a
137
Cs source (9 Gy). Group 2 received full thickness burn injury on 25% total body surface area followed by resuscitated with saline (2mL, IP). Group 3 received radiation followed 10 minutes later by burn injury. Group 4 were sham treated controls. After treatment, the mice were fasted for 23 hours and then injected (IV) with 50 µCi of
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FDG. One hour post injection, the mice were sacrificed and biodistribution was measured. Positive blood cultures were observed in all groups of animals compared to the shams. Increased mortality was observed after 6 days in the burn plus radiated group as compared to the other groups. Radiation and burn treatments separately or in combination produced major changes in
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FDG uptake by many tissues. In the heart, brown adipose tissue (BAT) and spleen, radiation plus burn produced a much greater increase (p<0.0001) in
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FDG accumulation than either treatment separately. All three treatments produced moderate decreases in
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FDG accumulation (p<0.01) in the brain and gonads. Burn injury, but not irradiation, increased
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FDG accumulation in skeletal muscle; however the combination of burn plus radiation decreased
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FDG accumulation in skeletal muscle. This model may be useful for understanding the effects of burns + irradiation injury on glucose metabolism and in developing treatments for victims of injuries produced by the combination of burn plus irradiation.