The characteristics of the caseinolytic activity of the contents of the large intestine in dysbiotic states

• Published in: Žurnal mikrobiologii, ėpidemiologii i immunobiologii no. 1; p. 80
• Main Authors: Kuzikov, A N, Bondarenko, V M, Lykova, E A, Stachenkova, S V, Shakhmardanov, M Z, Zemskova, L N, Isaeva, N P
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01-01-1998
• Subjects: Adolescent; Adult; Bacterial Infections - enzymology; Bacterial Infections - microbiology; Caseins - metabolism; Child; Child, Preschool; Feces - enzymology; Feces - microbiology; Female; Gastrointestinal Contents - enzymology; Gastrointestinal Contents - microbiology; Humans; Infant, Newborn; Infant, Premature; Intestinal Diseases - enzymology; Intestinal Diseases - microbiology; Intestine, Large - enzymology; Intestine, Large - microbiology; Linear Models; Male; Peptide Hydrolases - metabolism
• ISSN: 0372-9311

In the course of the study of the biochemical rapid method for the evaluation of microecological changes in the intestine by means of the test for the caseinolytic activity of fecal supernatants, the simultaneous presence of several enzymes in the samples under test was shown. Different degrees of the thermal inactivation of the active principle, linked with the expression of the positive caseinolytic sample, were established. The manifestations of caseinolytic activity, differing in their thermal stability, were found to have the proteolysis zone of equal size. The study showed the stability of this sign and its preservation during 1.5-2 years of storage of fecal supernatants in a refrigerator. Some details of making tests according to this method and the conditions ensuring the standard reproducibility of results were ascertained.