Use of a new antimacrophage monoclonal antibody D11 in diagnosis of hemoblastosis

• Published in: Gematologiia i transfuziologiia Vol. 40; no. 4; p. 13
• Main Authors: Frenkel', M A, Tupitsyn, N N, Rudinskaia, T D, Poltoranina, V S, Lebedeva, N B, Volkova, M A, Kaletin, G I
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01-07-1995
• Subjects: Adult; Antibodies, Monoclonal; Blast Crisis - diagnosis; Diagnosis, Differential; Humans; Macrophages - immunology
• ISSN: 0234-5730

Expression of new Mab D11 on blood and bone marrow cells was investigated in 85 hemoblastosis patients. In normals, antigen D11 is expressed on some monocytes and all tissue macrophages. D11 was noted on lymphoblasts of 5 out of 10 cases with B-cell ALL and of 1 case in B-lymphoid blast crisis of chronic myeloid leukemia. In T-ALL, ANLL, non-Hodgkin's lymphomas in leukemization stage, hairy cell leukemia and chronic lymphoid leukemia the cells were nonresponsive to Mab D11. Unlike D11 which have round nuclei, lymphoblasts D11+ have folded nuclei and more pronounced cytoplasmic basophilia. There were both B and myeloid antigens on D11+ blasts. ALL D11+ patients had extramedullary foci, more suppressed granulocytic and thrombocytic components of hemopoiesis, shorter remissions than those with ALL D11-.