Extracellular Vesicles Derived from SIPA1 high Breast Cancer Cells Enhance Macrophage Infiltration and Cancer Metastasis through Myosin-9

• Published in: Biology (Basel, Switzerland) Vol. 11; no. 4
• Main Authors: Feng, Lingyun, Weng, Jun, Yao, Chenguang, Wang, Ruyuan, Wang, Ning, Zhang, Yilei, Tanaka, Yoshimasa, Su, Li
• Format: Journal Article
• Language: English
• Published: Switzerland 31-03-2022
• Subjects: myosin-9; extracellular vesicles; breast cancer; macrophage; SIPA1
• ISSN: 2079-7737; 2079-7737

Tumour cell metastasis can be genetically regulated by proteins contained in cancer cell-derived extracellular vesicles (EVs) released to the tumour microenvironment. Here, we found that the number of infiltrated macrophages was positively correlated with the expression of signal-induced proliferation-associated 1 (SIPA1) in invasive breast ductal carcinoma tissues and MDA-MB-231 xenograft tumours. EVs derived from MDA-MB-231 cells (231-EVs) significantly enhanced macrophage migration, compared with that from
-knockdown MDA-MB-231 cells (231/si-EVs) both in vitro and in vivo. We revealed that SIPA1 promoted the transcription of
, which encodes myosin-9, and up-regulated the expression level of myosin-9 in breast cancer cells and their EVs. We also found that blocking myosin-9 by either down-regulating SIPA1 expression or blebbistatin treatment led to the suppression of macrophage infiltration. Survival analysis showed that breast cancer patients with high expression of
and
molecules had worse relapse-free survival (
= 0.028). In summary, SIPA1
breast cancer can enhance macrophage infiltration through EVs enriched with myosin-9, which might aggravate the malignancy of breast cancer.