Dp71 depleted HBE cells displayed increased DNA damage and apoptosis induced by H 2 O 2

• Published in: Cellular & molecular biology letters Vol. 24; p. 42
• Main Authors: Tan, Sichuang, Zhao, Shuai, Xiao, Xuefei, Xiao, Lan, Xie, Jinliang, Tan, Sipin
• Format: Journal Article
• Language: English
• Published: England 2019
• Subjects: Apoptosis; Cell Line; DNA - drug effects; DNA - metabolism; DNA Damage; DNA Repair; Dystrophin - metabolism; Epithelial Cells - drug effects; Epithelial Cells - metabolism; Focal Adhesion Kinase 1 - genetics; Focal Adhesion Kinase 1 - metabolism; Gene Expression Regulation; Humans; Hydrogen Peroxide - pharmacology; Hydrogen Peroxide - toxicity; Lamin Type B - genetics; Oxidative Stress; Phosphorylation; Protein Processing, Post-Translational; Rad51 Recombinase - genetics; Rad51 Recombinase - metabolism; FAK; RAD51; DNA damage; Dp71; Apoptosis
• ISSN: 1689-1392

Human bronchial epithelium (HBE)-Dp71 anti-sense(AS)cells with stably transfected Dp71 siRNA plasmids were prepared for further exploration of Dp71 biological traits in cells other than PC12. HBE-Dp71AS cells displayed increased DNA damage induced by H
O
. Apoptosis of HBE-Dp71AS cells induced by H
O
was increased via enhancing caspase 3, caspase 8 and caspase 9. HBE-Dp71AS cells also displayed decreased proliferation and clonogenic formation. RAD51 was proved to be a new binding partner of Dp71 by co-immunoprecipitation (Ip) and immunofluorescence. Reduced RAD51 mRNA and protein levels were observed in HBE-Dp71AS cells. Decreased lamin B1, focal adhesion kinase (FAK), phosphorylated focal adhesion kinase (p-FAK) and phosphorylated protein kinase B (p-AKT) were detected in the HBE-Dp71AS cells, which functioned together with RAD51 as the molecular explanations for the character alterations of HBE-Dp71AS cells.