The HRM-new molecular technique of detection of pharmaceutical resistance of micobacteria of tuberculosis

• Published in: Klinicheskaia laboratornaia diagnostika Vol. 59; no. 7; p. 62
• Main Authors: Lavrova, O I, Al'vares Figerova, M V, Tvorogova, M G
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01-07-2014
• Subjects: DNA Mutational Analysis - methods; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; Drug Resistance, Bacterial - genetics; Humans; Mycobacterium tuberculosis - chemistry; Mycobacterium tuberculosis - genetics; Nucleic Acid Denaturation
• ISSN: 0869-2084

The early detection of isolates of mycobacterium of tuberculosis resistant to anti-tuberculosis pharmaceuticals is ultimately needed for optimization of treatment scheme and prevention of propagation of resistant strains. The literature review presents analysis of data concerning the new, fast and inexpensive technique of detection of mutations in genes of mycobacterium of tuberculosis competent for multiple medicinal resistance. The molecular genetic technique HRM (high resolution melting curve analysis) is based on implementation of amplification of DNA fragments with subsequent analysis of their curves of melting with high resolution. The technique demonstrated its high sensitivity and specificity. In contrast with other molecular genetic techniques of detection of resistance of mycobacterium of tuberculosis, the makes it possible to detect all mutations in interested fragments of genes and to distinguish DNA of mutant and wild types in their mixture. This technique has no contamination danger. The HRM-analysis can become a basis for development of diagnostic kits with purpose to detect tuberculosis with multiple medicinal resistance.