KARBOSULFANIN GOeKKUSAGI ALABALIKLARIN (Oncorhynchus mykiss) ERITROSIT ASETILKOLINESTERAZ (AChE) ENZIM AKTIVITESINE ETKISI

• Published in: Journal of FisheriesSciences.com Vol. 5; no. 3; p. 240
• Main Author: Capkin, Erol
• Format: Journal Article
• Language: English
• Published: 01-01-2011
• Subjects: Oncorhynchus mykiss; MED, Black Sea
• ISSN: 1307-234X; 1307-234X

Detection of changes in enzyme activities has been used as a method to determine the toxic effects of pesticides or other toxic chemical. In this study, chronic effects of pesticide carbosulfan (250 g/L, EC) used commonly in the Eastern Black Sea Region on enzyme activity of rainbow trout (Oncorhynchus mykiss) were determined. For this purpose, rainbow trout (116.88 +/-21.69 g and 22.39 +/-1.40 cm) were acclimated to the laboratory conditions and exposed to carbosulfan for 60 days in flow-throuhgt system (6 L/h). According to data of preliminary tests, carbosulfan concentration of test water was designed as 35 mu g/L. During the chronic tests, erythrocyte acetylcholinesterase (AChE) activity of rainbow trout was measured and inhibition rate of enzyme activity was determined. Changes in enzyme activity of rainbow trout was sta-tistically significant (p<0.05). Increase of inhibition rate on AChE activity was up to 3rd week. Inhibition rates of AChE was determined as 41.32 % and it was determined that change on enzyme activity affected fish behavior.