Searching for monooxygenases and hydrolases in bacteria from an extreme environment; Searching for monooxygenases and hydrolases in bacteria from an extreme environment
Microbial oxidation potentials of extremophiles recovered from Pampo Sul oil field, Campos Basin, Brazil, in pure culture or in consortia, were investigated using high-throughput screening (HTS) and multibioreactions. Camphor (1), cis-jasmone (2), 2-methyl-cyclohexanone (3), 1,2-epoxyoctane (4), phe...
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Published in: | Applied microbiology and biotechnology Vol. 87; no. 1; pp. 319 - 329 |
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Main Authors: | , , , , , , , , |
Format: | Journal Article |
Language: | English |
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01-06-2010
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Abstract | Microbial oxidation potentials of extremophiles recovered from Pampo Sul oil field, Campos Basin, Brazil, in pure culture or in consortia, were investigated using high-throughput screening (HTS) and multibioreactions. Camphor (1), cis-jasmone (2), 2-methyl-cyclohexanone (3), 1,2-epoxyoctane (4), phenylethyl acetate (5), phenylethyl propionate (6), and phenylethyl octanoate (7) were used to perform multibioreaction assays. Eighty-two bacterial isolates were recovered from oil and formation water samples and those presenting outstanding activities in HTS assays were identified by sequencing their 16S rRNA genes. These results revealed that most microorganisms belonged to the genus Bacillus and presented alcohol dehydrogenase, monooxygenase, epoxide hydrolase, esterase, and lipase activities. |
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AbstractList | Microbial oxidation potentials of extremophiles recovered from Pampo Sul oil field, Campos Basin, Brazil, in pure culture or in consortia, were investigated using high-throughput screening (HTS) and multibioreactions. Camphor (1), cis-jasmone (2), 2-methyl-cyclohexanone (3), 1,2-epoxyoctane (4), phenylethyl acetate (5), phenylethyl propionate (6), and phenylethyl octanoate (7) were used to perform multibioreaction assays. Eighty-two bacterial isolates were recovered from oil and formation water samples and those presenting outstanding activities in HTS assays were identified by sequencing their 16S rRNA genes. These results revealed that most microorganisms belonged to the genus Bacillus and presented alcohol dehydrogenase, monooxygenase, epoxide hydrolase, esterase, and lipase activities. |
Author | Cruz, Georgiana F Vasconcellos, Suzan P Santos Neto, Eugenio V Angolini, Celio FF Oliveira, Valeria M Crespim, Elaine Oliveira, Luciana G Marsaioli, Anita J Lopes, Patricia F |
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Title | Searching for monooxygenases and hydrolases in bacteria from an extreme environment; Searching for monooxygenases and hydrolases in bacteria from an extreme environment |
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