Searching for monooxygenases and hydrolases in bacteria from an extreme environment; Searching for monooxygenases and hydrolases in bacteria from an extreme environment

Microbial oxidation potentials of extremophiles recovered from Pampo Sul oil field, Campos Basin, Brazil, in pure culture or in consortia, were investigated using high-throughput screening (HTS) and multibioreactions. Camphor (1), cis-jasmone (2), 2-methyl-cyclohexanone (3), 1,2-epoxyoctane (4), phe...

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Published in:Applied microbiology and biotechnology Vol. 87; no. 1; pp. 319 - 329
Main Authors: Cruz, Georgiana F, Angolini, Celio FF, Oliveira, Luciana G, Lopes, Patricia F, Vasconcellos, Suzan P, Crespim, Elaine, Oliveira, Valeria M, Santos Neto, Eugenio V, Marsaioli, Anita J
Format: Journal Article
Language:English
Published: 01-06-2010
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Abstract Microbial oxidation potentials of extremophiles recovered from Pampo Sul oil field, Campos Basin, Brazil, in pure culture or in consortia, were investigated using high-throughput screening (HTS) and multibioreactions. Camphor (1), cis-jasmone (2), 2-methyl-cyclohexanone (3), 1,2-epoxyoctane (4), phenylethyl acetate (5), phenylethyl propionate (6), and phenylethyl octanoate (7) were used to perform multibioreaction assays. Eighty-two bacterial isolates were recovered from oil and formation water samples and those presenting outstanding activities in HTS assays were identified by sequencing their 16S rRNA genes. These results revealed that most microorganisms belonged to the genus Bacillus and presented alcohol dehydrogenase, monooxygenase, epoxide hydrolase, esterase, and lipase activities.
AbstractList Microbial oxidation potentials of extremophiles recovered from Pampo Sul oil field, Campos Basin, Brazil, in pure culture or in consortia, were investigated using high-throughput screening (HTS) and multibioreactions. Camphor (1), cis-jasmone (2), 2-methyl-cyclohexanone (3), 1,2-epoxyoctane (4), phenylethyl acetate (5), phenylethyl propionate (6), and phenylethyl octanoate (7) were used to perform multibioreaction assays. Eighty-two bacterial isolates were recovered from oil and formation water samples and those presenting outstanding activities in HTS assays were identified by sequencing their 16S rRNA genes. These results revealed that most microorganisms belonged to the genus Bacillus and presented alcohol dehydrogenase, monooxygenase, epoxide hydrolase, esterase, and lipase activities.
Author Cruz, Georgiana F
Vasconcellos, Suzan P
Santos Neto, Eugenio V
Angolini, Celio FF
Oliveira, Valeria M
Crespim, Elaine
Oliveira, Luciana G
Marsaioli, Anita J
Lopes, Patricia F
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  fullname: Marsaioli, Anita J
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Bacillus
Title Searching for monooxygenases and hydrolases in bacteria from an extreme environment; Searching for monooxygenases and hydrolases in bacteria from an extreme environment
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