Cytochrome P-450 catalyzed oxidation of 1-piperidinoanthraquinone

• Published in: Law and contemporary problems Vol. 44; no. 4; pp. 634 - 639
• Main Authors: Vaĭner, L M, Iamkovoĭ, V I, Tsyrlov, I B, Pospelova, L N, Liakhovich, V V
• Format: Journal Article
• Language: Russian
• Published: 01-04-1979
• ISSN: 0320-9725

Oxidation of 1-piperidinoantraquinone (1-PA) in microsomal fractions of rat liver was studied. The only product of complete oxidation of 1-PA--(N-antraquinone-1)-delta-aminovaleric acid-was identified using paper and thin-layer chromatography. The participation of cytochrome P-450 in oxidation of 1-PA was demonstrated by sharp inhibition, involving blowing of the microsomes with CO and treatment with sodium deoxycholate. Studies of differential spectra of cytochrome P-450 in the presence of 1-PA are indicative of the first type of binding between 1-PA and cytochrome P-450. The binding constants (Ks) and the kinetic parameters (Km and V) for the above substrate in control microsomes and in those induced by phenobarbital and 3-methyl cholanthrene were determined. The results obtained suggest that cytochrome P-450 is involved in oxidation of a number of heterocyclic compounds resulting in the opening of the ring.