Paraprevotella clam gen. nov., sp. nov. and Paraprevotella xylaniphila sp. nov., members of the family 'Prevotellaceae' isolated from human faeces

• Published in: International journal of systematic and evolutionary microbiology Vol. 59; no. 8; pp. 1895 - 1900
• Main Authors: Morotomi, M, Nagai, F, Sakon, H, Tanaka, R
• Format: Journal Article
• Language: English
• Published: 01-08-2009
• Subjects: Prevotella
• ISSN: 1466-5026
• DOI: 10.1099/ijs.0.008169-0

Two anaerobic, non-spore-forming, pleomorphic, Gram-negative rods, designated YIT 11840 super(T) and YIT 11841 super(T), were isolated from human faeces. The organisms were catalase-negative, produced succinic and acetic acids as end products of glucose metabolism and had DNA G+C contents of approximately 48-49 mol%. Although the phenotypic characteristics of these two strains were very similar, analysis of their 16S rRNA gene sequences showed that they are only distantly related (93.8%), indicating that they represent two different species. A comparative sequence analysis revealed that these two species are members of the family 'Prevotellaceae' but are phylogenetically distant (<88% sequence similarity) from the known genera belonging to this family, including Prevotella, Hallela and Xylanibacter. On the basis of the phylogenetic analysis and physiological tests, strains YIT 11840 super(T) and YIT 11841 super(T) represent two novel species of a new genus, for which the names Paraprevotella clara gen. nov., sp. nov. (type strain YIT 11840 super(T) =JCM 14859 super(T)=DSM 19731 super(T)), the type species, and Paraprevotella xylaniphila sp. nov. (type strain YIT 11841 super(T) =JCM 14860 super(T) =DSM 19681 super(T)) are proposed.