Effect of Ag super(+) on membrane permeability of perfused Helix pomatia) neurons

Isolated, non-identified neurons were voltage clamped using the internal perfusion technique. Ions of Ag super(+) (1-100 mu M) introduced into the bathing solution activated a steady-state inward current (I sub(Ag)) in the soma. The effect of Ag super(+) was reversible when the concentration of Ag s...

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Bibliographic Details
Published in:	The Journal of physiology Vol. 442; pp. 1 - 13
Main Authors:	Gyori, J, Kiss, T, Shcherbatko, AD, Belan, P V, Tepikin, A V, Osipenko, ON, Salanki, J
Format:	Journal Article
Language:	English
Published:	01-01-1991
Subjects:	effects on Helix pomatia membranes neurons permeability silver
Online Access:	Get full text
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Summary:	Isolated, non-identified neurons were voltage clamped using the internal perfusion technique. Ions of Ag super(+) (1-100 mu M) introduced into the bathing solution activated a steady-state inward current (I sub(Ag)) in the soma. The effect of Ag super(+) was reversible when the concentration of Ag super(+) was less than 75 mu M or the time of application was shorter than 10 min. It is concluded that extracellularly applied Ag super(+) ions increase the cytoplasmic free Ca super(2+) concentration, which in turn activates non-specific cationic channels. Ag super(+) ions in 1-10 mu M concentration were able to decrease the voltage-activated Ca super(2+) current amplitude. This decrease, however, was due to the increase of (Ca super(2+)) sub(i) which caused Ca super(2+)-dependent inactivation.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 content type line 23 ObjectType-Feature-1
ISSN:	0022-3751