Studies of the association and conformational properties of metal-free insulin in alkaline sodium chloride solutions by one- and two-dimensional super(1)H NMR

One- and two-dimensional super(1)H NMR spectroscopy have been employed to probe the association and subsequent conformational changes of metal-free insulin in sodium chloride solution at pH 9 and 9.4. These studies establish that the proton resonances of His(B5) and His(B10) are useful signatures of...

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Bibliographic Details
Published in:	The Journal of biological chemistry Vol. 267; no. 13; pp. 8963 - 8970
Main Authors:	Kadima, W, Roy, M, Lee, RW-K, Kaarsholm, N C, Dunn, M F
Format:	Journal Article
Language:	English
Published:	01-01-1992
Subjects:	conformation insulin N.M.R sodium chloride solutions
Online Access:	Get full text
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Summary:	One- and two-dimensional super(1)H NMR spectroscopy have been employed to probe the association and subsequent conformational changes of metal-free insulin in sodium chloride solution at pH 9 and 9.4. These studies establish that the proton resonances of His(B5) and His(B10) are useful signatures of aggregation and conformation.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 content type line 23 ObjectType-Feature-2
ISSN:	0021-9258