NH sub(2)-terminal globular domain of human platelet glycoprotein Ib alpha has a methionine super(145)/threonine super(145) amino acid polymorphism, which is associated with the HPA-2 (Ko) alloantigens

• Published in: The Journal of clinical investigation Vol. 89; no. 2; pp. 381 - 384
• Main Authors: Kuijpers, RWAM, Faber, N M, Cuypers, HTM, Ouwehand, W H, von dem Borne, AEGK
• Format: Journal Article
• Language: English
• Published: 01-01-1992
• ISSN: 0021-9738

The glycoprotein (GP) Ib/IX complex, a prominent platelet GP complex, is the primary receptor for vWF. Previously, we have established that an antigenic polymorphism of platelets, the HPA-2 or Ko alloantigen system, is located on the 45-kD amino-terminal globular domain of GPIb alpha . With the polymerase chain reaction, we have amplified two segments of the GPIb alpha gene coding for the first 382 amino acids of two HPA-2a and two HPA-2b homozygous individuals. DNA-typing for the HPA-2 alloantigen system on genomic DNA obtained from a small number of cells may be applied for determining the genotype of a fetus from an immunized mother or of severely thrombocytopenic patients.