The human leukotriene A sub(4) hydrolase gene is expressed in two alternatively spliced mRNA forms
We identified a novel short (s-) mRNA of the human leukotriene-A sub(4) (LTA sub(4)) hydrolase using sequential reverse transcriptase PCR mapping. The s-mRNA is generated by skipping an 83 bp exon located in the 3' coding region and suggests the expression of an LTA sub(4) hydrolase isoform wit...
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| Published in: | Biochemical journal Vol. 314; no. 3; pp. 733 - 737 |
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| Main Authors: | , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
01-01-1996
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| Online Access: | Get full text |
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| Summary: | We identified a novel short (s-) mRNA of the human leukotriene-A sub(4) (LTA sub(4)) hydrolase using sequential reverse transcriptase PCR mapping. The s-mRNA is generated by skipping an 83 bp exon located in the 3' coding region and suggests the expression of an LTA sub(4) hydrolase isoform with a calculated molecular mass of 59 kDa and a distinct C-terminus. Both LTA sub(4) hydrolase mRNAs are constitutively expressed in blood cells, endothelial cells, smooth muscle cells, fibroblasts and tumour cells. The ratios of their mRNA expression levels are cell specific, with relatively high s-form mRNA expression in reticulocytes. Our data strongly suggest that the novel mRNA codes for the structurally related but distinct LTA sub(4) hydrolase isoenzyme that has been postulated. |
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| Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 content type line 23 ObjectType-Feature-1 |
| ISSN: | 0264-6021 |